The most important feature of an ori is the “Copy Number” - how many copies of the plasmid the cell will be commanded to make. The origin of replication is the region of plasmid DNA that the host organism uses to determine how many copies to make during everyday life and during reproduction. There appears to be a largely untraced peer-to-peer plasmid sharing community and you may be able to source plasmids from a friend without any cost.Įven narrowing down the world of plasmids to the AddGene catalogue can be a bit overwhelming, so this page will largely focus on a couple of my favourite plasmids īackbone Features: Origin of Replication (ori) It isn’t considered living or a GMO and can be legally shipped internationally. On the other hand, dried plasmid material on blotter paper appears to be a completely uncontrolled substance by international regulators. However shipping it within an organism makes it a controlled substance in some jurisdictions, so be sure to consult your national regulator before making an order! I also think that they have some of the most interesting and accessible educational resources available for free on their website, so be sure to explore it fully after reading this guide! AddGene traditionally ships plasmids inside living bacteria as an ‘agar stab’ which is a handy way to get a free strain of lab E. They charge a flat rate ($US 75) for plasmid sharing and are willing to deal with small labs that have a proven biosafety record. If you want a plasmid, there’s no better place to shop around than AddGene. It really cuts down on primer and restriction enzyme expenses. However there is nothing like a well quantified and reliable plasmid with a handily placed Multi-Cloning-Site (MCS) that you’re really familiar with. Plasmids are ubiquitous and infinitely varied in nature, so you’ve got plenty of options to choose from. If you came here from the GBlock Design page, you’re likely wondering which plasmid is going to be best for your experiment. This circular nature allows for “supercoiling” - whereby the plasmid gets wrapped up really tightly, small enough that it can slip through the cellular membranes and into the cytoplasm of cells. A plasmid is a circular sequence of double stranded DNA.
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